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Lung Histological Methods

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Lung Innate Immunity and Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1809))

Abstract

The lung is ideally suited to the application of histological methods to study its structure, cellular composition, and molecular characteristics of more than 30 types of cells. The key in these endeavors are proper tissue preservation/fixation, well-established protocols aimed at sectioning and staining, and understanding of lung morphology. Molecular studies can be performed in laser-captured cells and microscopic structures.

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References

  1. Weibel ER, Gomez DM (1962) Architecture of the human lung. Use of quantitative methods establishes fundamental relations between size and number of lung structures. Science 137:577–585

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  2. Crapo JD, Young SL, Fram EK, Pinkerton KE, Barry BE, Crapo RO (1983) Morphometric characteristics of cells in the alveolar region of mammalian lungs. Am Rev Respir Dis 128:S42–S46

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Author information

Authors and Affiliations

  1. Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA

    Aneta Gandjeva & Rubin M. Tuder

Authors
  1. Aneta Gandjeva
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  2. Rubin M. Tuder
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Corresponding author

Correspondence to Rubin M. Tuder .

Editor information

Editors and Affiliations

  1. Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, Colorado, USA

    Scott Alper

  2. Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, Colorado, USA

    William J. Janssen

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Gandjeva, A., Tuder, R.M. (2018). Lung Histological Methods. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8570-8_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8569-2

  • Online ISBN: 978-1-4939-8570-8

  • eBook Packages: Springer Protocols

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