Abstract
In this chapter, we describe methods for functional genomics studies in mouse macrophages. In particular, we describe complementary methods for gene inhibition using RNA interference (RNAi) and gene overexpression. These methods are readily amenable to medium- and high-throughput functional genomics investigations. These complementary loss-of-function and gain-of-function genomic approaches provide a rapid means of investigating the function of candidate genes prior to initiating more cumbersome studies in vivo.
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Acknowledgments
This work was funded by NIH grant R01ES025161 and the Wendy Siegel Fund for Leukemia and Cancer Research. Thanks to D. Golenbock for the Elam-Luc reporter construct.
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Lee, F.FY., Alper, S. (2018). Functional Genomics in Murine Macrophages. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_18
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DOI: https://doi.org/10.1007/978-1-4939-8570-8_18
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