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Lung Innate Immunity and Inflammation pp 169–201Cite as

Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1809))

Abstract

In the lungs, the epithelium is a first line of innate defense. In acute settings, such as infection or particulate exposure, the epithelium is protective. Protection is conferred by the epithelium’s role as a physical barrier and by its ability to synthesize proteins that promote defense directly through physical interactions (e.g., mucins and anti-microbial peptides) and indirectly through the production of proteins that regulate inflammation (e.g., cytokines and chemokines). Despite its importance as a first line of host defense, the epithelium is also a significant target and an effector in lung pathologies. Accordingly, to determine the significance and biological mechanisms of genes involved in pulmonary defense, it is important to be able to interrogate the lung epithelium. In mice, this presents challenges related to the cellular location and timing of interventions. Effective genetic strategies for targeting the lung epithelium using tissue-/cell-specific and inducible control have been developed over the past decade. Methods for spatiotemporal targeting of gene expression are described here.

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Authors and Affiliations

  1. Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver School of Medicine, Aurora, CO, USA

    Adrianne L. Stefanski, Dorota S. Raclawska & Christopher M. Evans

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  1. Adrianne L. Stefanski
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  2. Dorota S. Raclawska
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  3. Christopher M. Evans
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Correspondence to Christopher M. Evans .

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Editors and Affiliations

  1. Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, Colorado, USA

    Scott Alper

  2. Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, Colorado, USA

    William J. Janssen

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Stefanski, A.L., Raclawska, D.S., Evans, C.M. (2018). Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_14

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