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Rat Microglia Isolation and Characterization Using Multiparametric Panel for Flow Cytometric Analysis

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Pre-Clinical and Clinical Methods in Brain Trauma Research

Part of the book series: Neuromethods ((NM,volume 139))

Abstract

In the field of traumatic brain injury (TBI), characterization of microglia is traditionally done using imaging-based methods as immunohistochemistry (IHC), while flow cytometry (FC) is a supporting method that reassures the results. However, often, the flow cytometry method used, especially in the commonly used rat models, is not microglial specific, not comprehensive, and certainly not standardized. Here we describe the full, optimized procedure for isolating rat microglia, proper sample preparation for FC run and provide essential analysis guidelines. This protocol allows the specific identification of microglia, by discriminating them from other myeloid cells, with high-resolution observation of microglial subpopulations and their activation markers expression.

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Authors and Affiliations

  1. Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, Texas, USA

    Naama E. Toledano Furman, Karthik S. Prabhakara, Supinder Bedi, Charles S. Cox Jr. & Scott D. Olson

Authors
  1. Naama E. Toledano Furman
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  2. Karthik S. Prabhakara
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  3. Supinder Bedi
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  4. Charles S. Cox Jr.
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  5. Scott D. Olson
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Corresponding author

Correspondence to Naama E. Toledano Furman .

Editor information

Editors and Affiliations

  1. Department of Pediatric Surgery, McGovern Medical School, The University of Texas Health Sciences Center at Houston, Houston, Texas, USA

    Amit K. Srivastava

  2. Department of Pediatric Surgery, McGovern Medical School, The University of Texas Health Sciences Center at Houston, Houston, Texas, USA

    Charles S. Cox

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Toledano Furman, N.E., Prabhakara, K.S., Bedi, S., Cox, C.S., Olson, S.D. (2018). Rat Microglia Isolation and Characterization Using Multiparametric Panel for Flow Cytometric Analysis. In: Srivastava, A., Cox, C. (eds) Pre-Clinical and Clinical Methods in Brain Trauma Research. Neuromethods, vol 139. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8564-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8564-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8563-0

  • Online ISBN: 978-1-4939-8564-7

  • eBook Packages: Springer Protocols

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