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Analysis of Progranulin-Mediated Akt and MAPK Activation

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Progranulin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1806))

Abstract

Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models.

Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling.

In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.

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Acknowledgments

Our laboratories are supported by the Benjamin Perkins Bladder Cancer Fund, the Schorsch Family Fund for Innovative Medical Research and Care, National Institutes of Health Grants RO1 CA164462 (A.M., R.V.I.), Associazione Italiana per la Ricerca sul Cancro (AIRC) (grant n. 10625/12), AIRC project Calabria 2015, and Fondazione Cassa di Risparmio di Calabria e Lucania PON01_01078 (A.B.).

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Authors and Affiliations

  1. Biology of Prostate Cancer Program, Department of Urology, Thomas Jefferson University, Philadelphia, PA, USA

    Shi-Qiong Xu, Ryuta Tanimoto, Manuela Stefanello & Andrea Morrione

  2. Cancer Cell Biology and Signaling Program, Department of Pathology, Anatomy and Cell Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA

    Simone Buraschi & Renato V. Iozzo

  3. Department of Health and Endocrinology, University Magna Graecia of Catanzaro, Catanzaro, Italy

    Antonino Belfiore

Authors
  1. Shi-Qiong Xu
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  2. Simone Buraschi
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  3. Ryuta Tanimoto
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  4. Manuela Stefanello
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  5. Antonino Belfiore
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  6. Renato V. Iozzo
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  7. Andrea Morrione
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Corresponding author

Correspondence to Andrea Morrione .

Editor information

Editors and Affiliations

  1. Department of Medicine, McGill University MUHC, Montreal, Québec, Canada

    Andrew Bateman

  2. Department of Medicine, McGill University MUHC, Montreal, Québec, Canada

    Hugh P.J. Bennett

  3. Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China

    Siu Tim Cheung

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Xu, SQ. et al. (2018). Analysis of Progranulin-Mediated Akt and MAPK Activation. In: Bateman, A., Bennett, H., Cheung, S. (eds) Progranulin. Methods in Molecular Biology, vol 1806. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8559-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8559-3_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8557-9

  • Online ISBN: 978-1-4939-8559-3

  • eBook Packages: Springer Protocols

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