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Progranulin pp 305–313Cite as

Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-inflammatory Activity of Progranulin in Inflammatory Arthritis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1806))

Abstract

Progranulin (PGRN) was found to play an anti-inflammatory and protective role in both inflammatory and degenerative arthritis (Tang et al., Science 332:478–484, 2011; Zhao et al., Ann Rheum Dis 74:2244–2253, 2015). We recently published a visualized protocol to demonstrate a surgically-induced mouse model for examining the protective role of PGRN in degenerative osteoarthritis (Zhao et al., J Vis Exp:e50924, 2014). Herein we describe a modified collagen-induced arthritis (CIA) mouse model to investigate the anti-inflammatory activity of PGRN in inflammatory arthritis. CIA model is the most commonly used autoimmune model of inflammatory arthritis which shares both immunological and pathological features with human rheumatoid arthritis. Autoimmune inflammatory arthritis is induced by immunization with an emulsion of complete Freund’s adjuvant and chicken type II collagen (CII) using a modified procedure in PGRN deficient mice and control littermates. Using the protocol described here, the investigator should be able to reproducibly induce a high incidence of CIA in PGRN deficient mice and also learn how to critically evaluate the severity and incidence of this disease model.

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Acknowledgment

This work is supported partly by NIH research grants R01AR062207, R01AR061484, and R56AI100901, a Disease Targeted Research Grant from the American College of Rheumatology Research and Education Foundation.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, New York University Medical Center, New York, NY, USA

    Jian-Lu Wei & Chuan-Ju Liu

  2. Department of Cell Biology, New York University School of Medicine, New York, NY, USA

    Chuan-Ju Liu

Authors
  1. Jian-Lu Wei
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  2. Chuan-Ju Liu
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Corresponding author

Correspondence to Chuan-Ju Liu .

Editor information

Editors and Affiliations

  1. Department of Medicine, McGill University MUHC, Montreal, Québec, Canada

    Andrew Bateman

  2. Department of Medicine, McGill University MUHC, Montreal, Québec, Canada

    Hugh P.J. Bennett

  3. Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China

    Siu Tim Cheung

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Wei, JL., Liu, CJ. (2018). Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-inflammatory Activity of Progranulin in Inflammatory Arthritis. In: Bateman, A., Bennett, H., Cheung, S. (eds) Progranulin. Methods in Molecular Biology, vol 1806. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8559-3_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8559-3_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8557-9

  • Online ISBN: 978-1-4939-8559-3

  • eBook Packages: Springer Protocols

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