Abstract
With single-molecule localization microscopy (SMLM) it is possible to reveal the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. SMLM remains technically challenging, and frequently its implementation requires tailored experimental conditions that depend on the complexity of the subcellular structure of interest. Here, we describe two simple, robust, and high-throughput protocols to study molecular motors and machineries responsible for chromosome transport and organization in bacteria using 2D- and 3D-SMLM.
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Acknowledgments
We thank Andres Cardozo Gizzi for critical reading and helpful comments. This research was supported by funding from the European Research Council under the 7th Framework Program (FP7/2010-2015, ERC grant agreement 260787). We acknowledge support from France-BioImaging (FBI, ANR-10-INSB-04).
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Cattoni, D.I., Fiche, JB., Le Gall, A., Nollmann, M. (2018). DNA Organization and Superesolved Segregation. In: Lavelle, C. (eds) Molecular Motors. Methods in Molecular Biology, vol 1805. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8556-2_14
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DOI: https://doi.org/10.1007/978-1-4939-8556-2_14
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