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Synthesis of Photoactivatable and Paramagnetic Gangliosides

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Gangliosides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1804))

Abstract

For the physicochemical studies aimed at elucidating the dynamic of gangliosides in membranes and their interaction with proteins and membrane lipids, photoactivatable and paramagnetic ganglioside derivatives have proved to be invaluable tools. Here, protocols for the synthesis of such ganglioside derivatives are described. These derivatives bear in their ceramide portion either a highly photoreactive (3-trifluoromethyl)phenyldiazirinyl- or a spin active doxyl-labeled acyl chain in place of their natural acyl chain.

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Acknowledgments

The author’s work was supported by the Deutsche Forschungsgemeinschaft. Grants Schw 143/5-4 and SFB 284/B5, which hereby is gratefully acknowledged.

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Authors and Affiliations

  1. LIMES, c/o Kekulé-Institut f. Organische Chemie und Biochemie, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany

    Günter Schwarzmann

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  1. Günter Schwarzmann
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Correspondence to Günter Schwarzmann .

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Editors and Affiliations

  1. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Sandro Sonnino

  2. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Alessandro Prinetti

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Schwarzmann, G. (2018). Synthesis of Photoactivatable and Paramagnetic Gangliosides. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8552-4_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8551-7

  • Online ISBN: 978-1-4939-8552-4

  • eBook Packages: Springer Protocols

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