Abstract
The antibody preparation method to the glycolipid is basically same to the method to the protein. However, because the immunogenicity of the carbohydrate moieties of glycolipid is generally low in the mouse, the development of the anti-glycolipid antibody using purified glycolipid as an immunogen was not yet established. Here, we describe a method using a purified ganglioside adsorbed onto Salmonella minnesota for the efficient production of an anti-ganglioside mouse monoclonal antibody that recognizes the carbohydrate moieties of the ganglioside.
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Acknowledgment
We would like to thank Dr. Tadashi Tai and colleagues; most of these techniques were developed in collaboration with Dr. Tai’s laboratory.
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Yuasa, N., Kawashima, I. (2018). Methods for the Preparation of Anti-ganglioside Monoclonal Antibodies. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_13
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DOI: https://doi.org/10.1007/978-1-4939-8552-4_13
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