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Nuclear Magnetic Resonance of Gangliosides

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Gangliosides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1804))

Abstract

Structure, conformation, and dynamics of sphingolipids can provide substantial help in better understanding sphingolipid–ligand interaction mechanisms. Both the oligosaccharide structure and the ceramide moiety of native glycosphingolipid can be established directly by NMR spectroscopic analysis without the necessity to resort to any other chemical or spectroscopic methods. NMR is a powerful technique to investigate interaction between small ligand, such as ganglioside, and membrane protein.

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Abbreviations

1D:

One-dimensional

2D:

Two-dimensional

Cer:

Ceramide

COSY:

Correlation spectroscopy

DANTE:

Delays alternated by nutation for tailored excitation

Deac-GM1:

Neu-GM1

DEPT:

Distortionless enhancement by polarization transfer

DMPC:

1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine

DMPE:

Dimyristoyl phosphatidylethanolamine

DMPG:

1,2-Dimyristoyl-sn-glycero-3-phosphoglycerol

DMPS:

Dimyristoyl phosphatidylserine

DMSO:

Dimethyl-d 6 -sulfoxide

DOSY:

Diffusion Ordered Spectroscopy

DPC:

Dodecylphosphocholine

DPC:

Dodecylphosphocholine-d38;

EDTA:

Ethylenediaminetetraacetic acid

FA:

Fatty acid

Gal:

Galactose

GalNAc:

N-acetylgalactosamine

Glc:

Glucose

HMBC:

Heteronuclear Multiple Bond Correlation

HOHAHA:

Homonuclear Hartman Hahn

HSQC:

Heteronuclear Single Quantum Coherence

INEPT:

Insensitive nuclei enhanced by polarization transfer

MD:

Molecular dynamics

MINSY:

Mixing irradiation during NOESY

MLEV:

Malcolm LEVitt's decoupling cycle

Neu5Ac:

N-acetylneuraminic acid

Neu5Gc:

Neu5Gc N-glycolylneuraminic acid

NMR:

Nuclear Magnetic Resonance

NOE:

Nuclear Overhauser effect

NOESY:

NOE correlated spectroscopy

PCS:

Pseudocontact shift

PFG:

Pulsed Field Gradient

PFG-SE:

Pulsed Field Gradient Spin Echo

PRE:

Paramagnetic Relaxation Enhancement

ROE:

Rotating-frame nuclear Overhauser effect

ROESY:

Rotating-frame Overhauser Effect Spectroscopy

Sph:

Sphingosine

STD:

Saturation transfer difference

STEP:

Selective TOCSY edited preparation

SUV:

Small Unilamellar Vesicles

TOCSY:

Total correlated spectroscopy

TPPI:

Time proportional phase incrementation

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Authors and Affiliations

  1. Centro Interdipartimentale Misure, University of Parma, Parma, Italy

    Domenico Acquotti

  2. Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy

    Laura Mauri & Sandro Sonnino

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  1. Domenico Acquotti
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  2. Laura Mauri
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  3. Sandro Sonnino
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Correspondence to Domenico Acquotti .

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Editors and Affiliations

  1. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Sandro Sonnino

  2. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Alessandro Prinetti

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Acquotti, D., Mauri, L., Sonnino, S. (2018). Nuclear Magnetic Resonance of Gangliosides. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_12

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