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Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing

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Immunotoxicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1803))

Abstract

Innate lymphoid cells (ILCs) comprise a family of innate immune cells that orchestrate mucosal immune responses: initiating, sustaining, and even curbing immune responses. ILCs are relatively rare (≤1% of lymphocytes in mucosal tissues), lack classical cell-surface markers, and can be divided into three subsets (type 1-3 ILCs) based on differences in cytokine production, phenotype, and developmental pathway. Because ILCs can only be identified by combinations of cell-surface markers and cytokine production, multicolor flow cytometry is the most reliable method to purify, characterize, and assess the functionality of ILCs. Here, we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILCs from the lung.

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Author information

Authors and Affiliations

  1. Baker Institute for Animal Health and Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY, USA

    Elia D. Tait Wojno

  2. Department of Biomedical and Pharmaceutical Sciences, Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, MT, USA

    Celine A. Beamer

Authors
  1. Elia D. Tait Wojno
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  2. Celine A. Beamer
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Corresponding author

Correspondence to Celine A. Beamer .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, East Carolina University, Greenville, North Carolina, USA

    Jamie C. DeWitt

  2. Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA

    Cheryl E. Rockwell

  3. Durham, North Carolina, USA

    Christal C. Bowman

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Tait Wojno, E.D., Beamer, C.A. (2018). Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing. In: DeWitt, J., Rockwell, C., Bowman, C. (eds) Immunotoxicity Testing. Methods in Molecular Biology, vol 1803. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8549-4_21

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  • DOI: https://doi.org/10.1007/978-1-4939-8549-4_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8548-7

  • Online ISBN: 978-1-4939-8549-4

  • eBook Packages: Springer Protocols

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