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CD4+ T Cell Differentiation and Activation

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Immunotoxicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1803))

Abstract

The activation and differentiation of CD4+ T cells play a critical role in establishing and subsequently controlling protective adaptive immune responses. Flow cytometry is a powerful technique with which to assess the potential of xenobiotics to influence CD4+ T cell activation and differentiation. With flow cytometry, cells are stained with fluorochrome-conjugated antibodies and/or specific fluorescent probes to assess T cell activation, proliferation, effector cytokine production, and transcription factor expression. This technique allows for complex phenotypic analysis of tens to hundreds of thousands of individual cells very rapidly to assess the potential impact of a xenobiotic on CD4 effector differentiation and activation state.

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Author information

Authors and Affiliations

  1. Division of Biological Sciences, University of Montana, Missoula, MT, USA

    Jim Reed & Scott A. Wetzel

  2. Division of Biological Sciences, Center for Environmental Health Sciences, University of Montana, Missoula, MT, USA

    Scott A. Wetzel

Authors
  1. Jim Reed
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  2. Scott A. Wetzel
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Corresponding author

Correspondence to Scott A. Wetzel .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, East Carolina University, Greenville, North Carolina, USA

    Jamie C. DeWitt

  2. Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA

    Cheryl E. Rockwell

  3. Durham, North Carolina, USA

    Christal C. Bowman

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Reed, J., Wetzel, S.A. (2018). CD4+ T Cell Differentiation and Activation. In: DeWitt, J., Rockwell, C., Bowman, C. (eds) Immunotoxicity Testing. Methods in Molecular Biology, vol 1803. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8549-4_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8549-4_20

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8548-7

  • Online ISBN: 978-1-4939-8549-4

  • eBook Packages: Springer Protocols

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