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Flow Cytometry for the Immunotoxicologist

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Immunotoxicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1803))

Abstract

Assessing the immunotoxicity of xenobiotics by current regulatory testing has revealed compounds that can cause immunosuppression and stimulation. Flow cytometry is a cutting edge technique that can provide data on how toxicants can alter the quality and quantity of the immune response after exposure. Here we describe protocols for how to use flow cytometry to measure the immune response in multiple rodent organs (blood and lymphoid and nonlymphoid) as well as in novel models recently being utilized in the field of toxicology. These methods can be used for current testing and to determine mechanisms by which a xenobiotic can cause immunotoxicity.

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Author information

Authors and Affiliations

  1. Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA

    Scott T. Espenschied

  2. Department of Medicine, Duke University Medical Center, Durham, NC, USA

    Robert M. Tighe

  3. Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC, USA

    Kymberly M. Gowdy

Authors
  1. Scott T. Espenschied
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  2. Robert M. Tighe
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  3. Kymberly M. Gowdy
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Corresponding author

Correspondence to Kymberly M. Gowdy .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, East Carolina University, Greenville, North Carolina, USA

    Jamie C. DeWitt

  2. Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA

    Cheryl E. Rockwell

  3. Durham, North Carolina, USA

    Christal C. Bowman

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Espenschied, S.T., Tighe, R.M., Gowdy, K.M. (2018). Flow Cytometry for the Immunotoxicologist. In: DeWitt, J., Rockwell, C., Bowman, C. (eds) Immunotoxicity Testing. Methods in Molecular Biology, vol 1803. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8549-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8549-4_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8548-7

  • Online ISBN: 978-1-4939-8549-4

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