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HLA Typing pp 115-133 | Cite as

Super High Resolution for Single Molecule-Sequence-Based Typing of Classical HLA Loci Using Ion Torrent PGM

  • Takashi ShiinaEmail author
  • Shingo Suzuki
  • Jerzy K. Kulski
  • Hidetoshi Inoko
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1802)

Abstract

Super high resolution-single molecule-sequence-based typing (SS-SBT) is an HLA DNA typing method to the field 4 level of allelic resolution (formerly known as 8-digit typing) to efficiently detect novel and null alleles without phase ambiguity by combination of long ranged PCR amplification and next-generation sequencing (NGS) technologies. In this chapter, we describe three basic steps, long ranged PCR, NGS, and allele assignment.

Keywords

HLA Next-generation sequencing (NGS) Ion Torrent PGM long ranged polymerase chain reaction (PCR) Genotyping Super high resolution single molecule—sequence-based typing (SS-SBT) 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Takashi Shiina
    • 1
    Email author
  • Shingo Suzuki
    • 1
  • Jerzy K. Kulski
    • 1
    • 2
  • Hidetoshi Inoko
    • 1
  1. 1.Department of Molecular Life Science, Division of Basic Medical Science and Molecular MedicineTokai University School of MedicineIseharaJapan
  2. 2.School of Psychiatry and Clinical NeurosciencesThe University of Western AustraliaCrawleyAustralia

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