Abstract
The actions of thyroid hormones on brain development and function are due primarily to regulation of gene expression. Identification of direct transcriptional responses requires cell culture approaches given the difficulty of in vivo studies. Here, we describe the use of primary cells in culture obtained from embryonic mouse cerebral cortex, to identify the set of genes regulated directly and indirectly by T3 using RNA-Seq.
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Acknowledgments
Supported by grants SAF2014-54919-R from the Plan Estatal de Investigación Científica y Técnica y de Innovación, Spain and by the Center for Research on Rare Dieseases (Ciberer) under the frame of E-Rare-2, the ERA-Net for Research on rare Diseases. The contribution of Drs Pilar Gil-Ibañez and Mónica M. Belinchón is gratefully acknowledged.
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Bernal, J., Morte, B. (2018). Expression Analysis of Genes Regulated by Thyroid Hormone in Neural Cells. In: Plateroti, M., Samarut, J. (eds) Thyroid Hormone Nuclear Receptor. Methods in Molecular Biology, vol 1801. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7902-8_3
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DOI: https://doi.org/10.1007/978-1-4939-7902-8_3
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