Abstract
As the genome of experimental animals has become easier to manipulate, a number of mouse models have been developed to understand in vivo thyroid hormone action. A major site of thyroid hormone action is the HPT axis. While several methods are available that provide a detailed understanding of the HPT axis in mice, many authors choose to include only cursory data about this axis, which can lead to erroneous conclusions about in vivo thyroid hormone action. A standard protocol is proposed to evaluate the HPT axis in mice.
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References
Chiamolera MI, Wondisford FE (2009) Minireview: Thyrotropin-releasing hormone and the thyroid hormone feedback mechanism. Endocrinology 150(3):1091–1096
Costa-e-Sousa RH, Hollenberg AN (2012) Minireview: The neural regulation of the hypothalamic-pituitary-thyroid axis. Endocrinology 153(9):4128–4135
Ortiga-Carvalho TM, Sidhaye AR, Wondisford FE (2014) Thyroid hormone receptors and resistance to thyroid hormone disorders. Nat Rev Endocrinol 10(10):582–591
Ortiga-Carvalho TM, Chiamolera MI, Pazos-Moura CC, Wondisford FE (2016) Hypothalamus-pituitary-thyroid axis. Compr Physiol 6(3):1387–1428
Bianco AC, Anderson G, Forrest D, Galton VA, Gereben B, Kim BW, Kopp PA, Liao XH, Obregon MJ, Peeters RP, Refetoff S, Sharlin DS, Simonides WS, Weiss RE, Williams GR (2014) American Thyroid Association Guide to investigating thyroid hormone economy and action in rodent and cell models. Thyroid 1:88–168
Abel ED, Moura EG, Ahima RS, Campos-Barros A, Pazos-Moura CC, Boers ME, Kaulbach HC, Forrest D, Wondisford FE (2003) Dominant inhibition of thyroid hormone action selectively in the pituitary of thyroid hormone receptor-β null mice abolishes the regulation of thyrotropin by thyroid hormone. Mol Endocrinol 17(9):1767–1776
Nikrodhanond AA, Ortiga-Carvalho TM, Shibusawa N, Hashimoto K, Liao XH, Refetoff S, Yamada M, Mori M, Wondisford FE (2006) Dominant role of thyrotropin-releasing hormone in the hypothalamic-pituitary-thyroid axis. J Biol Chem 281(8):5000–5007
Machado DS, Sabet A, Santiago LA, Sidhaye AR, Chiamolera MI, Ortiga-Carvalho TM, Wondisford FE (2009) A thyroid hormone receptor mutation that dissociates thyroid hormone regulation of gene expression in vivo. Proc Natl Acad Sci U S A 106(23):9441–9446
Acknowledgment
These studies were supported by the National Institutes of Health (DK49126).
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Wondisford, F.E. (2018). Evaluating the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Mice. In: Plateroti, M., Samarut, J. (eds) Thyroid Hormone Nuclear Receptor. Methods in Molecular Biology, vol 1801. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7902-8_13
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DOI: https://doi.org/10.1007/978-1-4939-7902-8_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7902-8
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