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Metal Ion-Induced Self-Assembly and Packaging of CCMV Nanocapsules

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Protein Scaffolds

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1798))

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Abstract

The controlled self-assembly of protein cages is vital for the use of these nanocompartments in biomedical and nanotechnological applications. Recently, we showed that by combining different structural peptide elements, it is possible to assemble viral capsid proteins in distinct well-defined morphologies. In this chapter, a triblock copolypeptide is discussed, consisting of a metal ion-coordinating hexahistidine tag, a stimulus-responsive elastin-like polypeptide and a pH-responsive self-assembling viral capsid protein. This protein is able to form two different types of capsids, depending on the assembly pathway that is followed. Here, we focus on the metal ion-induced assembly process and describe the relevant experimental procedures to induce and utilize this assembly behavior.

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Acknowledgments

The Ministry of Education, Culture, and Science (Gravitation program 024.001.035) is acknowledged for financial support.

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Authors and Affiliations

  1. Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands

    Lise Schoonen & Mark B. van Eldijk

  2. Eindhoven University of Technology, Eindhoven, The Netherlands

    Jan C. M. van Hest

Authors
  1. Lise Schoonen
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  2. Mark B. van Eldijk
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  3. Jan C. M. van Hest
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Corresponding author

Correspondence to Jan C. M. van Hest .

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Editors and Affiliations

  1. Department of Chemistry, Occidental College, Los Angeles, California, USA

    Andrew K. Udit

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Schoonen, L., van Eldijk, M.B., van Hest, J.C.M. (2018). Metal Ion-Induced Self-Assembly and Packaging of CCMV Nanocapsules. In: Udit, A. (eds) Protein Scaffolds. Methods in Molecular Biology, vol 1798. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7893-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7893-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7892-2

  • Online ISBN: 978-1-4939-7893-9

  • eBook Packages: Springer Protocols

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