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Oxidative Stress Assessment in Zebrafish Larvae

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1797))

Abstract

Chemical or environmental aggression often leads to oxidative stress and antioxidant responses in organisms in which are involved several components and enzymes. Catalase, glutathione-S-transferase, total glutathione and lipid peroxidation are key elements to understand the oxidative status of an organism and have been measured using spectrophotometric methods adapted to 96-well microtiter plates. In this work we describe the methodologies for analyses in pools of 96 h zebrafish (Danio rerio) embryos.

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References

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Correspondence to Inês Domingues .

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Domingues, I., Gravato, C. (2018). Oxidative Stress Assessment in Zebrafish Larvae. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 1797. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7883-0_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7883-0_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7882-3

  • Online ISBN: 978-1-4939-7883-0

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