Abstract
Chemical or environmental aggression often leads to oxidative stress and antioxidant responses in organisms in which are involved several components and enzymes. Catalase, glutathione-S-transferase, total glutathione and lipid peroxidation are key elements to understand the oxidative status of an organism and have been measured using spectrophotometric methods adapted to 96-well microtiter plates. In this work we describe the methodologies for analyses in pools of 96 h zebrafish (Danio rerio) embryos.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Claiborne AL (1985) Catalase activity. CRC Handb Methods Oxyg Radic Res 1:283–284
Habig WH, Jakoby WB (1981) Assays for differentiation of glutathione S-transferases. Methods Enzymol 77:398–405
Tietze F (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522
Bird RP, Draper HH (1984) Comparative studies on different methods of malonaldehyde determination. Methods Enzymol 105:299–305
Abe FR, Gravato C, Soares AMVM, de Oliveira DP (2017) Biochemical approaches to assess oxidative stress induced by exposure to natural and synthetic dyes in early life stages in zebrafish. J Toxicol Environ Health A:1–10. https://doi.org/10.1080/15287394.2017.1371091
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 254:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
OECD (2013) Test No. 236: Fish embryo acute toxicity (FET) test, OECD guidelines for the testing of chemicals, Section 2. OECD Publ.:1–22
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Domingues, I., Gravato, C. (2018). Oxidative Stress Assessment in Zebrafish Larvae. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 1797. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7883-0_26
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7883-0_26
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7882-3
Online ISBN: 978-1-4939-7883-0
eBook Packages: Springer Protocols