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On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation Method

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Cellulases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1796))

Abstract

The conversion of renewable lignocellulosic biomass into fuels, chemicals, and high-value materials using the biochemical platform has been considered the most sustainable alternative for the implementation of future biorefineries. However, the high cost of the cellulolytic enzymatic cocktails used in the saccharification step significantly affects the economics of industrial large-scale conversion processes. The on-site production of enzymes, integrated to the biorefinery plant, is being considered as a potential strategy that could be used to reduce costs. In such approach, the microbial production of enzymes can be carried out using the same lignocellulosic biomass as feedstock for fungal development and biofuels production. Most of the microbial cultivation processes for the production of industrial enzymes have been developed using the conventional submerged fermentation. Recently, a sequential solid-state followed by submerged fermentation has been described as a potential alternative cultivation method for cellulolytic enzymes production. This chapter presents the detailed procedure of the sequential cultivation method, which could be employed for the on-site production of the cellulolytic enzymes required to convert lignocellulosic biomass into simple sugars.

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Acknowledgments

The author would like to thank Embrapa, CNPq (Process 401182/2014-2), CAPES, and FAPESP (Process 2014/19000-3 and 2016/10636-8) (all from Brazil) for financial support, and the students and technicians from Embrapa Instrumentation for their invaluable contribution.

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Authors and Affiliations

  1. Embrapa Instrumentation, São Carlos, SP, Brazil

    Cristiane S. Farinas & Camila Florencio

  2. Graduate Program of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil

    Cristiane S. Farinas & Alberto C. Badino

Authors
  1. Cristiane S. Farinas
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  2. Camila Florencio
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  3. Alberto C. Badino
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Corresponding author

Correspondence to Cristiane S. Farinas .

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Editors and Affiliations

  1. Section of Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University, Copenhagen, Denmark

    Mette Lübeck

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Farinas, C.S., Florencio, C., Badino, A.C. (2018). On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation Method. In: Lübeck, M. (eds) Cellulases. Methods in Molecular Biology, vol 1796. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7877-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7877-9_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7876-2

  • Online ISBN: 978-1-4939-7877-9

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