Abstract
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes that oxidize the most recalcitrant polysaccharides and require extracellular electron donors. The role of electron donation to redox enzymes is pivotal since a nonefficient electron transfer might result in partial activity or reduced kinetics. In this protocol we show the effect of using excited photosynthetic pigments combined with reducing agents as efficient electron donors for monooxygenases. The light-induced electron transfer can enhance the oxidation ability of LPMOs up to ten times.
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Cannella, D. (2018). Light-Induced Electron Transfer Protocol for Enzymatic Oxidation of Polysaccharides. In: Lübeck, M. (eds) Cellulases. Methods in Molecular Biology, vol 1796. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7877-9_17
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DOI: https://doi.org/10.1007/978-1-4939-7877-9_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7876-2
Online ISBN: 978-1-4939-7877-9
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