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Plant Chemical Genomics pp 1–7Cite as

Multi-Parametric Screening in Arabidopsis thaliana Seedlings

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1795))

Abstract

Phenotypic screening and subsequent target identification approaches are very valuable to identify chemical probes that can be used to explore the connection between phenotypes and biological pathways. However, assessing a phenotypic effect in plants in a high-throughput fashion is a challenging task and often requires expensive readout devices. In this chapter, we describe a cost-effective multi-parametric screening procedure that is compatible with liquid-handling systems and that enables the assessment of phenotypes in Arabidopsis thaliana seedlings in an automated way.

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References

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Author information

Authors and Affiliations

  1. VIB Screening Core, Ghent, Belgium

    Long Nguyen, Andrzej Drozdzecki & Dominique Audenaert

  2. Expertise Centre for Bioassay Development and Screening (C-BIOS), Ghent University, Ghent, Belgium

    Vera Goossens & Dominique Audenaert

  3. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Bert De Rybel & Tom Beeckman

  4. VIB Center for Plant Systems Biology, Ghent, Belgium

    Bert De Rybel & Tom Beeckman

Authors
  1. Long Nguyen
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  2. Andrzej Drozdzecki
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  3. Vera Goossens
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  4. Bert De Rybel
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  5. Tom Beeckman
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  6. Dominique Audenaert
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Corresponding author

Correspondence to Dominique Audenaert .

Editor information

Editors and Affiliations

  1. Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA

    Friedrich Fauser

  2. Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA

    Martin Jonikas

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Nguyen, L., Drozdzecki, A., Goossens, V., De Rybel, B., Beeckman, T., Audenaert, D. (2018). Multi-Parametric Screening in Arabidopsis thaliana Seedlings. In: Fauser, F., Jonikas, M. (eds) Plant Chemical Genomics. Methods in Molecular Biology, vol 1795. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7874-8_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7874-8_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7873-1

  • Online ISBN: 978-1-4939-7874-8

  • eBook Packages: Springer Protocols

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