Abstract
The bimolecular fluorescence complementation (BiFC) assay is a powerful, flexible, and simple tool to study protein–protein interactions in living cells. To accelerate the production and assessment of BiFC constructs, Gateway-compatible multicolor BiFC vectors were generated to enable the simultaneous production of multiple fusion genes that have the split N- or C-terminal fragment of fluorescent protein with the gene of interest in a high-throughput manner. Two different transient expression techniques for the assessment of BiFC in plant cells are described.
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Acknowledgments
This work was supported by JSPS KAKENHI Grant Number JP15J40032 to Shino Goto-Yamada, JP26440157 to Shoji Mano, and JP16085101 and JP22120001 to Mikio Nishimura and Research Program on Hepatitis from Japan Agency for Medical Research and Development, AMED, to Mikio Nishimura. We thank the Model Plant Research Facility at the National Institute for Basic Biology for plant growth support, and the Spectrography and Bioimaging Facility and Functional Genomics Facility, NIBB Core Research Facilities, for technical support.
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Goto-Yamada, S., Hikino, K., Nishimura, M., Nakagawa, T., Mano, S. (2018). Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein–Protein Interactions in Plant Cells. In: Oñate-Sánchez, L. (eds) Two-Hybrid Systems. Methods in Molecular Biology, vol 1794. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7871-7_16
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DOI: https://doi.org/10.1007/978-1-4939-7871-7_16
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