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Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma

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Multiple Myeloma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1792))

Abstract

The application of fluorescence in situ hybridization (FISH) technology in diagnosis and molecular classification of cancer-risk has become an essential tool in the proceeding of personalized therapy. In multiple myeloma, the precise FISH detection of numerical and structural genetic aberrations can be carried out on metaphase chromosome spreads, interphase nuclei, and formalin fixed paraffin-embedded (FFPE) tissues. To dissect highly complex cancer genomes, a broad variety of novel DNA probes, which outpace supplies from commercial resources on the market, are also crucial to the advanced translational researches. Here, we provide the protocols for the creation of custom-made DNA probes and for conducting hybridizations on various targeting cells and tissues.

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Author information

Authors and Affiliations

  1. Myeloma Institute, University of Arkansas for Medical Sciences, Little Rock, AR, USA

    Erming Tian

Authors
  1. Erming Tian
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Correspondence to Erming Tian .

Editor information

Editors and Affiliations

  1. Janssen R&D, Spring House, PA, USA

    Christoph Heuck

  2. The Myeloma Institute, University of Arkansas for Medical Science, Little Rock, AR, USA

    Niels Weinhold

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Tian, E. (2018). Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma. In: Heuck, C., Weinhold, N. (eds) Multiple Myeloma. Methods in Molecular Biology, vol 1792. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7865-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7865-6_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7864-9

  • Online ISBN: 978-1-4939-7865-6

  • eBook Packages: Springer Protocols

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