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DRG Neuron/Schwann Cells Myelinating Cocultures

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Myelin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1791))

Abstract

Our understanding of the processes controlling peripheral nervous system myelination have been significantly benefited by the development of an in vitro myelinating culture system in which primary Schwann cells are cocultured together with primary sensory neurons. In this chapter, we describe the protocol currently used in our laboratories to establish Schwann cells neuronal myelinating cocultures. We also include a detailed description of the various substrates that can be used to establish it.

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Acknowledgments

We would like to thank Dr. James L. Salzer (New York University) for instrumental training on the world of myelinating Schwann cells neuronal cocultures.

We are also grateful to Dr. Steven Einheber (Hunter College New York), Dr. Angelo Quattrini (San Raffaele Scientific Institute), and Dr. Stefano Previtali (San Raffaele Scientific Institute) for providing pictures and for long-standing collaboration. Finally, we would like to thank present and past members of our laboratories for their many contributions to the studies cited here. Works in A.B. laboratory is supported by Telethon-Italy (GGP12017 and GGP15012A) and the ERA-Net for research programs on rare diseases (E-Rare 2). Works in C.T. laboratory is supported by Telethon Italy (GGP14040 and GGP15012) and the Italian Minister of Health (PE 13-9-T).

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Authors and Affiliations

  1. Division of Neuroscience and INSPE, San Raffaele Scientific Institute, Milan, Italy

    Carla Taveggia & Alessandra Bolino

Authors
  1. Carla Taveggia
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  2. Alessandra Bolino
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Corresponding authors

Correspondence to Carla Taveggia or Alessandra Bolino .

Editor information

Editors and Affiliations

  1. Nerve Disorders Laboratory, CIC bioGUNE, Derio, Vizcaya, Spain

    Ashwin Woodhoo

  2. Basque Foundation for Science, IKERBASQUE, Bilbao, Vizcaya, Spain

    Ashwin Woodhoo

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Taveggia, C., Bolino, A. (2018). DRG Neuron/Schwann Cells Myelinating Cocultures. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7862-5_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7861-8

  • Online ISBN: 978-1-4939-7862-5

  • eBook Packages: Springer Protocols

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