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Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry

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Myelin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1791))

Abstract

Lipids are essential components of cells and tissues. They play active and central roles in signaling and many biological functions and therefore their dysregulation is very often the first signal of function alteration. Here we describe the protocol to analyze not only lipid expression in rat sciatic nerve but also the lipid distribution along its different anatomic areas. The protocol combines results from MALDI-IMS and UHPLC-MS/MS to identify and cartography the maximum number of lipid species in the tissue.

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Acknowledgments

Work in the lab of the authors was funded by grants from the Basque Government (IT-971-16 and KK2016-00036 to BO) and the Spanish Ministry of Innovation, Economy and Competitiveness (SAF2015-64352 to BO and RTC-2015-3693-1 to JAF and BO). Technical and human support provided by the Servicio de Lipidómica of the SGIKER (UPV/EHU, MICINN, GV/EJ, ESF) is gratefully acknowledged. JG thanks the UPV/EHU for a pre-doctoral fellowship.

Author information

Authors and Affiliations

  1. Faculty of Science and Technology, Department of Physical Chemistry, University of the Basque Country (UPV/EHU), Leioa, Spain

    Roberto Fernández, Jone Garate & José A. Fernández

  2. Faculty of Science and Technology, Central Analysis Service, University of the Basque Country (UPV/EHU), Leioa, Spain

    Beatriz Abad

  3. Faculty of Medicine and Nursing, Department of Physiology, University of the Basque Country (UPV/EHU), Leioa, Spain

    Begoña Ochoa

Authors
  1. Roberto Fernández
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  2. Jone Garate
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  3. Beatriz Abad
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  4. Begoña Ochoa
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  5. José A. Fernández
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Corresponding author

Correspondence to José A. Fernández .

Editor information

Editors and Affiliations

  1. Nerve Disorders Laboratory, CIC bioGUNE, Derio, Vizcaya, Spain

    Ashwin Woodhoo

  2. Basque Foundation for Science, IKERBASQUE, Bilbao, Vizcaya, Spain

    Ashwin Woodhoo

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Fernández, R., Garate, J., Abad, B., Ochoa, B., Fernández, J.A. (2018). Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7862-5_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7861-8

  • Online ISBN: 978-1-4939-7862-5

  • eBook Packages: Springer Protocols

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