Abstract
Activity-dependent myelination modulates neuron conduction velocity and as such it is essential for a correct wiring of a whole nervous system. Increasing myelination through inducing neuron activity has been proposed as a treatment strategy for demyelination diseases. Yet, the mechanisms and the effects of activity-dependent myelination remain elusive—new tools are needed. In this chapter, we describe a novel compartmentalized device integrated with an optogenetic stimulator for studying activity-dependent myelination in vitro. The platform can be modified to include multiple cell types, stimulation modes, and experimental readouts to answer a specific research question. This versatility combined with a precise control over spatial extent of the stimulation and the stimulation pattern make the proposed platform a valuable tool for molecular myelination studies.
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Neto E, Leitao L, Sousa DM, Alves CJ, Alencastre IS, Aguiar P, Lamghari M (2016) Compartmentalized microfluidic platforms: the unrivaled breakthrough of in vitro tools for neurobiological research. J Neurosci 36(46):11573–11584. https://doi.org/10.1523/JNEUROSCI.1748-16.2016
Park J, Koito H, Li J, Han A (2009) Microfluidic compartmentalized co-culture platform for CNS axon myelination research. Biomed Microdevices 11(6):1145–1153. https://doi.org/10.1007/s10544-009-9331-7
Kilinc D, Blasiak A, Lee GU (2015) Microtechnologies for studying the role of mechanics in axon growth and guidance. Front Cell Neurosci 9:282. https://doi.org/10.3389/fncel.2015.00282
Prasad A, Teh DBL, Blasiak A, Chai C, Wu Y, Gharibani PM, Yang IH, Phan TT, Lim KL, Yang H, Liu X, All AH (2017) Static magnetic field stimulation enhances oligodendrocyte differentiation and secretion of neurotrophic factors. Sci Rep 7(1):6743. https://doi.org/10.1038/s41598-017-06331-8
Kilinc D, Blasiak A, Baghban M, Carville N, Al-Adli A, Al-Shammari R, Rice J, Lee G, Gallo K, Rodriguez B (2017) Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons. Appl Phys Lett 110:053702. https://doi.org/10.1063/1.4975304
Malone M, Gary D, Yang IH, Miglioretti A, Houdayer T, Thakor N, McDonald J (2013) Neuronal activity promotes myelination via a cAMP pathway. Glia 61(6):843–854. https://doi.org/10.1002/glia.22476
Yang IH, Gary D, Malone M, Dria S, Houdayer T, Belegu V, McDonald JW, Thakor N (2012) Axon myelination and electrical stimulation in a microfluidic, compartmentalized cell culture platform. NeuroMolecular Med 14(2):112–118. https://doi.org/10.1007/s12017-012-8170-5
Blasiak A, Lee HU, Nag S, Yang IH (2016) Compartmentalized microfluidic platform integrated with subcellular electrical stimulation for studying activity-dependent axon myelination. In: 2016 international conference on optical MEMS and nanophotonics (OMN), Singapore, 31 July–4 August 2016
Lee HU, Blasiak A, Agrawal DR, Loong DTB, Thakor NV, All AH, Ho JS, Yang IH (2017) Subcellular electrical stimulation of neurons enhances the myelination of axons by oligodendrocytes. PLoS One 12(7):e0179642. https://doi.org/10.1371/journal.pone.0179642
Mandal R, Nag S, Thakor NV (2015) Wirelessly powered and controlled, implantable, multi-channel, multi-wavelength optogenetic stimulator. In: IEEE MTT-S international microwave workshop series on RF and wireless technologies for biomedical and healthcare applications (IMWS-BIO), Singapore, 9–11 December 2014, p 1–3
Nag S, Lee P, Herikstad R, Sng J, Yen SC, Thakor NV (2015) Multi-function optogenetic stimulator and neural amplifier for wirelessly controlled neural interface. In: IEEE biomedical circuits and systems conference (BioCAS), Atlanta, Georgia, 22–24 October 2015. IEEE, p 1–4
Lee HU, Nag S, Blasiak A, Jin Y, Thakor N, Yang IH (2016) Subcellular optogenetic stimulation for activity-dependent myelination of axons in a novel microfluidic compartmentalized platform. ACS Chem Neurosci 7(10):1317–1324. https://doi.org/10.1021/acschemneuro.6b00157
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD (2006) Astrocytes promote myelination in response to electrical impulses. Neuron 49(6):823–832. https://doi.org/10.1016/j.neuron.2006.02.006
Blasiak A, Kilinc D, Lee GU (2016) Neuronal cell bodies remotely regulate axonal growth response to localized Netrin-1 treatment via second messenger and DCC dynamics. Front Cell Neurosci 10:298. https://doi.org/10.3389/fncel.2016.00298
Kilinc D, Blasiak A, O'Mahony JJ, Lee GU (2014) Low piconewton towing of CNS axons against diffusing and surface-bound repellents requires the inhibition of motor protein-associated pathways. Sci Rep 4:7128. https://doi.org/10.1038/srep07128
Shiroma LS, Piazzetta MH, Duarte-Junior GF, Coltro WK, Carrilho E, Gobbi AL, Lima RS (2016) Self-regenerating and hybrid irreversible/reversible PDMS microfluidic devices. Sci Rep 6:26032. https://doi.org/10.1038/srep26032
Bhattacharya S, Datta A, Berg JM, Gangopadhyay S (2005) Studies on surface wettability of poly(dimethyl) siloxane (PDMS) and glass under oxygen-plasma treatment and correlation with bond strength. J Microelectromech Syst 14(3):590–597. https://doi.org/10.1109/JMEMS.2005.844746
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Blasiak, A., Nag, S., Hong Yang, I. (2018). Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_16
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DOI: https://doi.org/10.1007/978-1-4939-7862-5_16
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7861-8
Online ISBN: 978-1-4939-7862-5
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