Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase

  • Anne E. Gibbons
  • Kathryn E. Luker
  • Gary D. LukerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1790)


Bioluminescence imaging is a powerful, broadly utilized method for noninvasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies of protein-protein interactions, ligand-receptor interactions, cell trafficking, and drug targeting in mouse models. We describe use of a novel luciferase enzyme derived from Oplophorus gracilirostris, NanoLuc, in cell-based assays bioluminescence imaging of tumor-bearing mice. We also combine NanoLuc with another luciferase enzyme, firefly luciferase, to image multiple signal transduction events in one imaging session.

Key words

NanoLuc Bioluminescence imaging Firefly luciferase Oplophorus gracilirostris 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Anne E. Gibbons
    • 1
  • Kathryn E. Luker
    • 1
  • Gary D. Luker
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Radiology, Center for Molecular ImagingUniversity of MichiganAnn ArborUSA
  2. 2.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA
  3. 3.Department of Microbiology and ImmunologyUniversity of MichiganAnn ArborUSA

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