Abstract
The presence, growth, or decline of transfected cell populations expressing the enzyme Luciferase can be followed in live mice using bioluminescence optical imaging techniques. This protocol describes how to verify the imaging equipment, options for injecting the substrate Luciferin into mice, image acquisition considerations, and commonly used data analysis techniques.
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Acknowledgments
This work was made possible by numerous faculty, student, and staff members at the UCLA Crump Institute for Molecular Imaging Preclinical Technology Imaging Center. Waldemar Ladno, DVM and Darin Williams were invaluable with veterinary and logistical help with operations, education, and research work.
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Stout, D., David, J. (2018). Optical Bioluminescence Protocol for Imaging Mice. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_3
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DOI: https://doi.org/10.1007/978-1-4939-7860-1_3
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7860-1
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