Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging

  • Ajit Dhadve
  • Bhushan Thakur
  • Pritha RayEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1790)


Dual modality reporter genes are powerful means of tracking cellular processes in cell culture systems and whole animals. In this chapter, we describe the methods for construction of a plasmid reporter gene vector expressing a fluorescent and a bioluminescent gene and its validation by in vitro assays in mammalian cells as well as by noninvasive imaging methods in small animal models.

Key words

Dual modality Optical imaging Bioluminescent Fluorescent Reporter gene Two-step transcriptional activator 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Imaging Cell Signaling & Therapeutics LabTata Memorial Centre (TMC), Advanced Centre for Treatment, Research and Education in Cancer (ACTREC)Navi MumbaiIndia
  2. 2.Homi Bhabha National InstituteMumbaiIndia
  3. 3.Homi Bhabha National InstituteMumbaiIndia

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