Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging
Dual modality reporter genes are powerful means of tracking cellular processes in cell culture systems and whole animals. In this chapter, we describe the methods for construction of a plasmid reporter gene vector expressing a fluorescent and a bioluminescent gene and its validation by in vitro assays in mammalian cells as well as by noninvasive imaging methods in small animal models.
Key wordsDual modality Optical imaging Bioluminescent Fluorescent Reporter gene Two-step transcriptional activator
- 8.Dobrucki LW et al (2010) Approaches to multimodality imaging of angiogenesis. J Nucl Med 1(51 Suppl):669–79SGoogle Scholar