Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models

  • Jessica Swanner
  • Ravi SinghEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1790)


Silver nanoparticles (AgNPs) have a high affinity for sulfhydryl (thiol) groups, which can be exploited for functionalization with various tracking and targeting moieties. Here, we describe how to reliably and reproducibly functionalize AgNPs with the fluorescent moiety cyanine3-polyethelyne glycol (5000 molecular weight)-thiol (Cy3-PEG5000-SH). We also demonstrate how to purify and characterize Cy3-functionalized AgNPs (Cy3-AgNPs). Additionally, we describe how these Cy3-AgNPs can be imaged in 2D and 3D tumor models, providing insight into cellular localization and diffusion through a tumor spheroid, respectively.

Key words

Silver Nanomaterials Fluorescence Imaging 3D tumor models 



This work was supported in part by grant R00CA154006 (RS) from the National Institutes of Health, pilot funds from the Comprehensive Cancer Center of Wake Forest University supported by NCI CCSG P30CA012197, and by start-up funds from the Wake Forest School of Medicine Department of Cancer Biology. JS was supported in part by training grant T32CA079448 from the National Institutes of Health.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Cancer BiologyWake Forest School of MedicineWinston SalemUSA
  2. 2.Comprehensive Cancer Center of Wake Forest Baptist Medical CenterWinston SalemUSA

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