Silver nanoparticles (AgNPs) have a high affinity for sulfhydryl (thiol) groups, which can be exploited for functionalization with various tracking and targeting moieties. Here, we describe how to reliably and reproducibly functionalize AgNPs with the fluorescent moiety cyanine3-polyethelyne glycol (5000 molecular weight)-thiol (Cy3-PEG5000-SH). We also demonstrate how to purify and characterize Cy3-functionalized AgNPs (Cy3-AgNPs). Additionally, we describe how these Cy3-AgNPs can be imaged in 2D and 3D tumor models, providing insight into cellular localization and diffusion through a tumor spheroid, respectively.
Silver Nanomaterials Fluorescence Imaging 3D tumor models
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This work was supported in part by grant R00CA154006 (RS) from the National Institutes of Health, pilot funds from the Comprehensive Cancer Center of Wake Forest University supported by NCI CCSG P30CA012197, and by start-up funds from the Wake Forest School of Medicine Department of Cancer Biology. JS was supported in part by training grant T32CA079448 from the National Institutes of Health.
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