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Reporter Gene Imaging pp 197–208Cite as

Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1790))

Abstract

Cerenkov luminescence imaging (CLI) is a relatively new imaging modality that utilizes conventional optical imaging instrumentation to detect Cerenkov radiation derived from standard and often clinically approved radiotracers. Its research versatility, low cost, and ease of use have increased its popularity within the molecular imaging community and at institutions that are interested in conducting radiotracer-based molecular imaging research, but that lack the necessary resources and infrastructure. Here, we provide a description of the materials and procedures necessary to conduct a Cerenkov luminescence imaging experiment using a variety of imaging instrumentation, radionuclides, and animal models.

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Acknowledgments

This work was supported by NIH grant NIH P30 CA012197, DoD grant W81XWH-13-1-0125, and The United States Department of Energy Office of Science-Isotope Program in the Office of Nuclear Physics.

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Authors and Affiliations

  1. Department of Cancer Biology, Wake Forest University Health Sciences, Winston-Salem, NC, USA

    Nikunj B. Bhatt, Darpan N. Pandya, Frank C. Marini, William H. Gmeiner & Thaddeus J. Wadas

  2. Department of Radiation Oncology, Wake Forest University Health Sciences, Winston-Salem, NC, USA

    William A. Dezarn

  3. Department of Biomedical Engineering, Wake Forest University Health Sciences, Winston-Salem, NC, USA

    Dawen Zhao

  4. Department of Hematology and Oncology, Wake Forest University Health Sciences, Winston-Salem, NC, USA

    Pierre L. Triozzi

Authors
  1. Nikunj B. Bhatt
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  2. Darpan N. Pandya
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  3. William A. Dezarn
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  4. Frank C. Marini
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  5. Dawen Zhao
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  6. William H. Gmeiner
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  7. Pierre L. Triozzi
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  8. Thaddeus J. Wadas
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Corresponding author

Correspondence to Thaddeus J. Wadas .

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Editors and Affiliations

  1. Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA

    Purnima Dubey

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Bhatt, N.B. et al. (2018). Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7860-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7858-8

  • Online ISBN: 978-1-4939-7860-1

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