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Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo

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Reporter Gene Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1790))

Abstract

Positron emission tomography (PET) is a three dimensional imaging modality that detects the accumulation of radiolabeled isotopes in vivo. Ectopic expression of a thymidine kinase reporter gene allows for the specific detection of reporter cells in vivo by imaging with the reporter specific probe. PET reporter imaging is sensitive, quantitative and can be scaled into larger tumors or animals with little to no tissue diffraction. Here, we describe how thymidine kinase PET reporter genes can be used to noninvasively image cancer cells in vivo.

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Acknowledgment

M.N.M. is supported by the Stanford Dean’s Fellowship and the A.P. Giannini Foundation.

Author information

Authors and Affiliations

  1. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA

    Melissa N. McCracken

  2. Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, CA, USA

    Melissa N. McCracken

  3. Stanford Cancer Institute, Stanford University School of Medicine, Stanford, CA, USA

    Melissa N. McCracken

Authors
  1. Melissa N. McCracken
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Editor information

Editors and Affiliations

  1. Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA

    Purnima Dubey

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McCracken, M.N. (2018). Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7860-1_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7858-8

  • Online ISBN: 978-1-4939-7860-1

  • eBook Packages: Springer Protocols

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