Abstract
Reporter gene-based molecular imaging is a powerful means to detect the movement and function of diverse cell populations in vivo. Reconstitution of an immune system marked with molecular imaging reporter genes permits tracking of primary immune responses to pathogens and cancer in experimental systems. This chapter describes the methods to isolate bone marrow stem/progenitors, transduce them with imaging reporter genes, and track the reconstitution of the peripheral immune system by bioluminescent imaging.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Cao YA et al (2004) Shifting foci of hematopoiesis during reconstitution from single stem cells. Proc Natl Acad Sci U S A 101:221–226. https://doi.org/10.1073/pnas.2637010100
Ahn SB et al (2017) Multimodality imaging of bone marrow-derived dendritic cell migration and antitumor immunity. Transl Oncol 10:262–270. https://doi.org/10.1016/j.tranon.2017.01.003
Edinger M et al (2003) Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging. Blood 101:640–648. https://doi.org/10.1182/blood-2002-06-1751
Shu CJ et al (2005) Visualization of a primary anti-tumor immune response by positron emission tomography. Proc Natl Acad Sci U S A 102:17412–17417. https://doi.org/10.1073/pnas.0508698102
Mitsunobu H, Teramoto J, Nishida K et al (2017) Beyond native Cas9: manipulating genomic information and function. Trends Biotechnol 35(10):983–996. https://doi.org/10.1016/j.tibtech.2017.06.004
Ellmeier W, Sunshine MJ, Losos K et al (1997) An enhancer that directs lineage-specific expression of CD8 in positively selected thymocytes and mature T cells. Immunity 7:537–547
Ogata H et al (1995) Long-term repopulation of hematolymphoid cells with only a few hemopoietic stem cells in mice. Proc Natl Acad Sci U S A 92:5945–5949
Grahn D, Hamilton KF (1957) Genetic variation in the acute lethal response of four inbred mouse strains to whole body X-irradiation. Genetics 42:189–198
Stewart CC, Stewart SJ (2004) Multiparameter data acquisition and analysis of leukocytes. Methods Mol Biol 263:45–66. https://doi.org/10.1385/1-59259-773-4:045
Acknowledgments
This work was supported by NIH 1 R21 CA124457 to P.D.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dubey, P. (2018). Visualization of Immune Cell Reconstitution by Bioluminescent Imaging. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_10
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7860-1_10
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7858-8
Online ISBN: 978-1-4939-7860-1
eBook Packages: Springer Protocols