Advertisement

Visualization of Immune Cell Reconstitution by Bioluminescent Imaging

  • Purnima DubeyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1790)

Abstract

Reporter gene-based molecular imaging is a powerful means to detect the movement and function of diverse cell populations in vivo. Reconstitution of an immune system marked with molecular imaging reporter genes permits tracking of primary immune responses to pathogens and cancer in experimental systems. This chapter describes the methods to isolate bone marrow stem/progenitors, transduce them with imaging reporter genes, and track the reconstitution of the peripheral immune system by bioluminescent imaging.

Key words

Luciferase Bone marrow Lymphocytes Imaging 

Notes

Acknowledgments

This work was supported by NIH 1 R21 CA124457 to P.D.

References

  1. 1.
    Cao YA et al (2004) Shifting foci of hematopoiesis during reconstitution from single stem cells. Proc Natl Acad Sci U S A 101:221–226.  https://doi.org/10.1073/pnas.2637010100CrossRefPubMedGoogle Scholar
  2. 2.
    Ahn SB et al (2017) Multimodality imaging of bone marrow-derived dendritic cell migration and antitumor immunity. Transl Oncol 10:262–270.  https://doi.org/10.1016/j.tranon.2017.01.003CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Edinger M et al (2003) Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging. Blood 101:640–648.  https://doi.org/10.1182/blood-2002-06-1751CrossRefPubMedGoogle Scholar
  4. 4.
    Shu CJ et al (2005) Visualization of a primary anti-tumor immune response by positron emission tomography. Proc Natl Acad Sci U S A 102:17412–17417.  https://doi.org/10.1073/pnas.0508698102CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Mitsunobu H, Teramoto J, Nishida K et al (2017) Beyond native Cas9: manipulating genomic information and function. Trends Biotechnol 35(10):983–996.  https://doi.org/10.1016/j.tibtech.2017.06.004CrossRefPubMedGoogle Scholar
  6. 6.
    Ellmeier W, Sunshine MJ, Losos K et al (1997) An enhancer that directs lineage-specific expression of CD8 in positively selected thymocytes and mature T cells. Immunity 7:537–547CrossRefPubMedGoogle Scholar
  7. 7.
    Ogata H et al (1995) Long-term repopulation of hematolymphoid cells with only a few hemopoietic stem cells in mice. Proc Natl Acad Sci U S A 92:5945–5949CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Grahn D, Hamilton KF (1957) Genetic variation in the acute lethal response of four inbred mouse strains to whole body X-irradiation. Genetics 42:189–198PubMedPubMedCentralGoogle Scholar
  9. 9.
    Stewart CC, Stewart SJ (2004) Multiparameter data acquisition and analysis of leukocytes. Methods Mol Biol 263:45–66.  https://doi.org/10.1385/1-59259-773-4:045CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Microbial Infection and ImmunityThe Ohio State UniversityColumbusUSA

Personalised recommendations