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Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana

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Plant Vacuolar Trafficking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1789))

Abstract

Eukaryotic cells comprise various organelles surrounded by the membrane. Each organelle is characterized by unique proteins and lipids and has its own specific functions. Single membrane-bounded organelles, including the Golgi apparatus, endosomes, and vacuoles are connected by membrane trafficking. Identifying the organelle localization of a protein of interest is essential for determining the proteins physiological functions. Here, we describe methods for determining protein subcellular localization using the inhibitors brefeldin A and wortmannin in Arabidopsis thaliana.

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Acknowledgments

This work was supported by Grant-in-Aid for JSPS Research Fellow to J.T. (15J07657) and Grants-in-Aid for Scientific Research to T.U. (No. 15H04627) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan

    Junpei Takagi & Tomohiro Uemura

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  1. Junpei Takagi
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  2. Tomohiro Uemura
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Correspondence to Tomohiro Uemura .

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  1. Faculty of Sciences, University of Porto, Porto, Portugal

    Cláudia Pereira

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Takagi, J., Uemura, T. (2018). Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana . In: Pereira, C. (eds) Plant Vacuolar Trafficking. Methods in Molecular Biology, vol 1789. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7856-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7856-4_12

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  • Print ISBN: 978-1-4939-7855-7

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