Functional Studies on Steroid Receptors

  • Simon Schlanger
  • Hannelore V. HeemersEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1786)


Nuclear receptors play an important role in prostate cancer and the androgen receptor is a key transcription factor in regulation of cellular events. Androgen receptor-associated coregulators may be upregulated or downregulated in prostate cancer. Altered expression of regulators may potentiate androgen-induced proliferation, migration, and invasion. Therapies aimed to modulate the function of coregulators in prostate cancer may be based on the use of small molecule inhibitors. Expression and function of AR-associated proteins could be investigated after overexpression and gene silencing followed by hormonal treatment, real-time RT-PCR and ChIP.

Key words

Androgen receptor Coregulatory proteins Transcriptional complexes Prostate cancer progression 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Cancer Biology, Cleveland ClinicLerner Research InstituteClevelandUSA
  2. 2.Department of Urology, Cleveland ClinicGlickman Urologic & Kidney InstituteClevelandUSA
  3. 3.Department of Hematology/Medical Oncology, Cleveland ClinicTaussig Cancer InstituteClevelandUSA

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