Angiogenesis is one important hallmark of cancer progression which explains the relevance of developing methods to efficiently analyze the neo-angiogenic process. In this report we make use of the transgenic adenocarcinoma of the murine prostate (TRAMP) model, considered a good model for studying clinical prostate cancer progression, to describe in detail the methods used to study angiogenesis in this type of solid tumor development. In this report we provide step-by-step procedures on the basis of previous work in our laboratory for: the mouse urogenital sinus (UGS) collection; microdissection of the prostate; preparation of the prostatic samples for immunofluorescence (to analyze vascular density, morphology, maturation, functionality, hypoxia, and others); preparation of prostatic samples to histopathological analysis and/or immunohistochemistry; and endothelial and vascular mural cell sorting and isolation by fluorescent associated cell sorting (FACS) to further analysis (mRNA, protein, or other) or to maintain in culture.
Prostate cancer TRAMP Tumor angiogenesis Endothelial and vascular mural cells
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
World Health Organization (2014) World cancer report, 2014, WHO report. WHO, GenevaGoogle Scholar
Taylor RA, Toivanen R, Risbridger GP (2010) Stem cells in prostate cancer: treating the root of the problem. Endocr Related Cancer 17:R273–R285CrossRefGoogle Scholar
Hayward SW, Rosen MA, Cunha GR (1997) Stromal-epithelial interactions in the normal and neoplastic prostate. Br J Urol 79(Suppl 2):18–26CrossRefPubMedGoogle Scholar
Russo G, Mischi M, Scheepens W et al (2012) Angiogenesis in prostate cancer: onset, progression and imaging. BJU Int 110:E794–E808CrossRefPubMedGoogle Scholar
Borre M, Offersen BV, Nerstrøm B et al (1998) Microvessel density predicts survival in prostate cancer patients subjected to watchful waiting. Br J Cancer 78:940–944CrossRefPubMedPubMedCentralGoogle Scholar
Bono AV, Celato N, Cova V et al (2002) Microvessel density in prostate carcinoma. Pros Cancer Pros Dis 5:123–127CrossRefGoogle Scholar
Papetti M, Herman IM (2002) Mechanisms of normal and tumor-derived angiogenesis. Am J Physiol Cell Physiol 282:C947–C970CrossRefPubMedGoogle Scholar
Kaplan-Lefko PJ, Chen T-M, Ittmann MM et al (2003) Pathobiology of autochthonous prostate cancer in a pre-clinical transgenic mouse model. Prostate 55:219–237CrossRefPubMedGoogle Scholar
Gratton JP, Lin MI, Yu J et al (2003) Selective inhibition of tumor microvascular permeability by cavtratin blocks tumor progression in mice. Cancer Cell 4:31–39CrossRefPubMedGoogle Scholar
Pedrosa A-R, Trindade A, Carvalho C et al (2015) Endothelial Jagged1 promotes solid tumor growth through both pro-angiogenic and angiocrine functions. Oncotarget 6:24404–24423CrossRefPubMedPubMedCentralGoogle Scholar