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Methods to Study Angiogenesis in a Mouse Model of Prostate Cancer

  • Ana-Rita Pedrosa
  • Alexandre Trindade
  • António DuarteEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1786)

Abstract

Angiogenesis is one important hallmark of cancer progression which explains the relevance of developing methods to efficiently analyze the neo-angiogenic process. In this report we make use of the transgenic adenocarcinoma of the murine prostate (TRAMP) model, considered a good model for studying clinical prostate cancer progression, to describe in detail the methods used to study angiogenesis in this type of solid tumor development. In this report we provide step-by-step procedures on the basis of previous work in our laboratory for: the mouse urogenital sinus (UGS) collection; microdissection of the prostate; preparation of the prostatic samples for immunofluorescence (to analyze vascular density, morphology, maturation, functionality, hypoxia, and others); preparation of prostatic samples to histopathological analysis and/or immunohistochemistry; and endothelial and vascular mural cell sorting and isolation by fluorescent associated cell sorting (FACS) to further analysis (mRNA, protein, or other) or to maintain in culture.

Key words

Prostate cancer TRAMP Tumor angiogenesis Endothelial and vascular mural cells 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ana-Rita Pedrosa
    • 1
  • Alexandre Trindade
    • 1
    • 2
  • António Duarte
    • 1
    • 2
    Email author
  1. 1.Faculdade de Medicina Veterinaria – ULisboa, Av. da Universidade Tecnica, Centro Interdisciplinar de Investigação em Sanidade Animal (CIISA)University of LisbonLisbonPortugal
  2. 2.Instituto Gulbenkian de CiênciaOeirasPortugal

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