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NMR-Based Prostate Cancer Metabolomics

  • Leslie R. Euceda
  • Maria K. Andersen
  • May-Britt Tessem
  • Siver A. Moestue
  • Maria T. Grinde
  • Tone F. BathenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1786)

Abstract

Prostate cancer is the second most common malignancy, and the fifth leading cause of cancer-related death among men, worldwide. A major unsolved clinical challenge in prostate cancer is the ability to accurately distinguish indolent cancer types from the aggressive ones. Reprogramming of metabolism is now a widely accepted hallmark of cancer development, where cancer cells must be able to convert nutrients to biomass while maintaining energy production. Metabolomics is the large-scale study of small molecules, commonly known as metabolites, within cells, biofluids, tissues, or organisms. Nuclear magnetic resonance (NMR) spectroscopy is commonly applied in metabolomics studies of cancer. This chapter provides protocols for NMR-based metabolomics of cell cultures, biofluids (serum and urine), and intact tissue, with concurrent advice for optimal biobanking and sample preparation procedures.

Key words

Biobanking Biofluids analysis Cell extracts analysis Metabolite quantification Metabolomics NMR pulse sequences NMR spectroscopy Prostate cancer Sample preparation Targeted metabolic pathway analysis Tissue analysis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Leslie R. Euceda
    • 1
  • Maria K. Andersen
    • 1
  • May-Britt Tessem
    • 1
  • Siver A. Moestue
    • 2
    • 3
  • Maria T. Grinde
    • 1
  • Tone F. Bathen
    • 4
    Email author
  1. 1.Department of Circulation and Medical ImagingNTNU - The Norwegian University of Science and TechnologyTrondheimNorway
  2. 2.Department of Clinical and Molecular MedicineNTNU - The Norwegian University of Science and TechnologyTrondheimNorway
  3. 3.Department of Pharmacy, Faculty of Health SciencesNord UniversityBodøNorway
  4. 4.Department of Circulation and Medical ImagingNTNU - The Norwegian University of Science and TechnologyTrondheimNorway

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