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Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer

  • Chris W. D. Armstrong
  • Oksana Lyubomska
  • Melissa J. LaBonte
  • David J. J. WaughEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1786)

Abstract

Cancer studies have entered an era that is heavily focused on the contribution of the tumor microenvironment. For this reason, in vivo experimentation in an immunodeficient model system is no longer fit for purpose. As a consequence, numerous genetically engineered mouse models (GEMMs) which self-develop tumors have been developed to allow experiments to be performed in a fully immunocompetent setting. One of the most commonly used technologies is Cre-loxP recombination due to its unique ability to control target gene expression in a specified tissue type. However, the major limitation of these models remains the inability to generate sufficient numbers of age-matched mice for a synchronized experimental start date. For this reason, the derivation of cell lines from genetically modified murine prostate tissue is desirable and allows for the generation of syngeneic models via subcutaneous or orthotopic injection.

Key words

Prostate cancer GEMMs Cell line derivation Cre-loxP recombination Mouse prostate dissection Genotyping Syngeneic 

Notes

Acknowledgements

The authors would like to acknowledge the CCRCB laboratory personnel who have helped to optimize these protocols; in particular, we would like to acknowledge Dr. Adam Pickard, Dr. Sharon Eddie Parkinson, and Dr. Lara Dura Perez for their work on cell line derivation from the mouse prostate tissue.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Chris W. D. Armstrong
    • 1
  • Oksana Lyubomska
    • 1
  • Melissa J. LaBonte
    • 1
  • David J. J. Waugh
    • 1
    Email author
  1. 1.Centre for Cancer Research and Cell Biology (CCRCB)Queen’s University BelfastBelfastUK

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