Abstract
The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.
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Acknowledgements
The Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, is supported by core funding from the Wellcome Trust [grant number 203128/Z/16/Z]. MAT is supported by core funding from the Manchester Collaborative Centre for Inflammation Research, the Medical Research Council and the Biotechnology and Biological Sciences Research Council. AMG is supported by the National Science Centre, Poland (POLONEZ fellowship UMO-2015/19/P/NZ7/03659).
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Kelly, A., Grabiec, A.M., Travis, M.A. (2018). Culture of Human Monocyte-Derived Macrophages. In: Rousselet, G. (eds) Macrophages. Methods in Molecular Biology, vol 1784. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7837-3_1
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DOI: https://doi.org/10.1007/978-1-4939-7837-3_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7836-6
Online ISBN: 978-1-4939-7837-3
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