Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString® nCounter® Platform

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1783)

Abstract

The NanoString nCounter Analysis System uses a digital fluorescent barcode technology that allows for direct multiplexed measurement of gene expression (mRNA), DNA, and protein. The technology uses molecular barcodes and single-molecule imaging to detect and count unique mRNA and protein targets in a single reaction. nCounter-based detection is enzyme-free (no amplification of mRNA is required), fully automated, and allows simultaneous detection of up to 770 mRNA and 30 protein targets from multiple sample types. Target counting is fully digital with quantitative data output. Here we describe preparation of solid tumor lysate samples for use in the nCounter Analysis System.

Key words

nCounter mRNA Proteins Antibodies Barcodes NanoString Digital counting 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.NanoString TechnologiesSeattleUSA

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