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Differential Gene Expression Analysis of Plants

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Gene Expression Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1783))

Abstract

Since the next-generation sequencing (NGS) systems were invented and introduced to life science research about a decade ago, the NGS technology has extensively utilized in wide range of genomic, transcriptomic, and evolutionary studies. Compared with other eukaryotic species, the application of NGS technology in plant research reveals some challenges in sample preparation and data analysis due to some structural and physiological characteristics and genome complexity nature in plants. Hence, despite of the standard sample preparation and data process protocols widely used in high throughput transcriptomic analysis, we also describe the modified hot borate RNA extraction protocol specific for high quality and quantity plant total RNA isolation, and some comments and suggestions to achieve better assessments in the validation of RNA and library quality and data analysis.

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Author information

Authors and Affiliations

  1. Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS, USA

    Mark Arick II

  2. Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS, USA

    Chuan-Yu Hsu

Authors
  1. Mark Arick II
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  2. Chuan-Yu Hsu
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Corresponding author

Correspondence to Mark Arick II .

Editor information

Editors and Affiliations

  1. Division of Geriatrics and Clinical Gerontology, National Institute on Aging, Bethesda, Maryland, USA

    Nalini Raghavachari

  2. Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, Mississippi, USA

    Natàlia Garcia-Reyero

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Arick, M., Hsu, CY. (2018). Differential Gene Expression Analysis of Plants. In: Raghavachari, N., Garcia-Reyero, N. (eds) Gene Expression Analysis. Methods in Molecular Biology, vol 1783. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7834-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7834-2_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7833-5

  • Online ISBN: 978-1-4939-7834-2

  • eBook Packages: Springer Protocols

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