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Mitochondrial Bioenergetics pp 157–170Cite as

Measurement of Proton Leak in Isolated Mitochondria

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1782))

Abstract

Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton leaks. In this chapter, we present a method to measure proton leak activity in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasized.

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Acknowledgments

C.A. thanks Julie Buckingham for the useful advice on the preparation of TPMP sleeves.

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Authors and Affiliations

  1. School of Biomedical and Healthcare Sciences, University of Plymouth, Plymouth, UK

    Charles Affourtit

  2. Buck Institute for Research on Aging, Novato, CA, USA

    Hoi-Shan Wong & Martin D. Brand

Authors
  1. Charles Affourtit
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  2. Hoi-Shan Wong
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  3. Martin D. Brand
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Corresponding author

Correspondence to Martin D. Brand .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos M. Palmeira

  2. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    António J. Moreno

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Affourtit, C., Wong, HS., Brand, M.D. (2018). Measurement of Proton Leak in Isolated Mitochondria. In: Palmeira, C., Moreno, A. (eds) Mitochondrial Bioenergetics. Methods in Molecular Biology, vol 1782. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7831-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7831-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7830-4

  • Online ISBN: 978-1-4939-7831-1

  • eBook Packages: Springer Protocols

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