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Mitochondrial Bioenergetics pp 315–324Cite as

Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1782))

Abstract

Mitochondrial autophagy or mitophagy is a process that selectively degrades mitochondria via autophagy. It is believed that mitophagy degrades damaged or unnecessary mitochondria and is important for maintaining mitochondrial homeostasis. To date, it is known that several stimuli can induce mitophagy. However, some of these stimuli (including iron depletion, hypoxia, and nitrogen starvation) induce mild mitophagy, which is difficult to detect by measuring the decrease in mitochondrial mass. Recently, we have successfully detected mitophagy induced under these conditions using mito-Keima as a reporter. In this chapter, we describe the protocols for induction and detection of iron depletion- and hypoxia-induced mitophagy using the mito-Keima-expressing cells.

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Acknowledgment

This work was supported in part by the Japan Society for the Promotion of Science KAKENHI [Grant numbers 17H03671 (T.K.), 16H01198 (T.K.), 16H01384 (T.K.), and 17 K15088 (S.Y.)], Yujin Memorial Grant (Niigata University School of Medicine) (T.K.), and Takeda Science Foundation (S.Y. and T.K.).

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Authors and Affiliations

  1. Department of Cellular Physiology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Shun-ichi Yamashita & Tomotake Kanki

Authors
  1. Shun-ichi Yamashita
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  2. Tomotake Kanki
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Correspondence to Tomotake Kanki .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos M. Palmeira

  2. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    António J. Moreno

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Yamashita, Si., Kanki, T. (2018). Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells. In: Palmeira, C., Moreno, A. (eds) Mitochondrial Bioenergetics. Methods in Molecular Biology, vol 1782. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7831-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7831-1_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7830-4

  • Online ISBN: 978-1-4939-7831-1

  • eBook Packages: Springer Protocols

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