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Mitochondrial Bioenergetics by 13C–NMR Isotopomer Analysis

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Mitochondrial Bioenergetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1782))

Abstract

Metabolic reprogramming has been associated to a plethora of diseases, and there has been increased demand for methodologies able to determine the metabolic alterations that characterize the pathological states and help developing metabolically centered therapies. In this chapter, methodologies for monitoring TCA cycle turnover and its interaction with pyruvate cycling and anaplerotic reactions will be presented. These methodologies are based in the application of stable 13C isotope “tracers”/substrates and 13C–NMR isotopomer analysis of metabolic intermediates. These methodologies can be applied at several organizational levels, ranging from isolated organelles and organs to whole organisms/humans. For the sake of simplicity, only very simple and well-defined models will be presented, including isolated heart mitochondria and isolated perfused hearts and livers.

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Author information

Authors and Affiliations

  1. Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal

    Rui A. Carvalho

  2. Centre for Functional Ecology, University of Coimbra, Coimbra, Portugal

    Rui A. Carvalho & Ivana Jarak

Authors
  1. Rui A. Carvalho
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  2. Ivana Jarak
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Corresponding author

Correspondence to Rui A. Carvalho .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos M. Palmeira

  2. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    António J. Moreno

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Carvalho, R.A., Jarak, I. (2018). Mitochondrial Bioenergetics by 13C–NMR Isotopomer Analysis. In: Palmeira, C., Moreno, A. (eds) Mitochondrial Bioenergetics. Methods in Molecular Biology, vol 1782. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7831-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7831-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7830-4

  • Online ISBN: 978-1-4939-7831-1

  • eBook Packages: Springer Protocols

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