Abstract
The amyloid -β peptide (Aβ) is the main component of the amyloid plaques in Alzheimer’s disease (AD). It has been widely demonstrated that Aβ is toxic to neurons and is associated with AD pathology. However, Aβ also appears to have an important biological function both in the adult brain and throughout embryonic development of the nervous system, acting as a trophic factor at low concentrations.
It is known that Neural Stem Cells (NSCs) are capable of self-renewal and differentiate into functional glial and neuronal cells. Therefore, human NSCs may be a hope for future therapeutic application in neurodegenerative diseases such as AD. The effects of Aβ peptides on NSCs are still not well understood and remain controversial.
In this chapter we outline the materials and methods used for the culture and differentiation of hNS1 cells, a cell line of human NSCs. We describe the preparation of different forms (monomeric, oligomeric and fibrillary) of Aβ peptide and subsequent cell treatment, followed by the analysis of the effects on toxicity, cell proliferation and cell fate specification of hNS1 cells.
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References
Zhang YW, Thompson R, Zhang H et al (2011) APP processing in Alzheimer's disease. Mol Brain 4:3
Peña-Casanova J (1999) Enfermedad de Alzheimer. Del diagnóstico a la terapia: conceptos y hechos. Barcelona: Fundación “La Caixa”
Serrano-Pozo A, Frosch MP, Masliah E et al (2011) Neuropathological alterations in Alzheimer disease. Cold Spring Harb Perspect Med 1(1):a006189
Manzano-León N, Mas-Oliva J (2006) Estrés oxidativo, péptido β-amiloide y enfermedad de Alzheimer. Gac Med Mex 142(3):229–238
Hardy J, Selkoe DJ (2002) The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics. Science 297(5580):353–356
Pérez V (2014) Identificación de regiones clave en el proceso de agregación y citotoxicidad del péptido β-amiloide. Tesis de máster. Universidad Autónoma de Nuevo León. Departamento de Bioquímica y Medicina Molecular, México
Dahlgren KN, Manelli AM, Stine WB Jr et al (2002) Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability. J Biol Chem 277(35):32046–32053
Pearson HA, Peers C (2016) Physiological roles for amyloid beta peptides. J Physiol 575(Pt 1):5–10
Cárdenas-Aguayo MC, Silva-Lucero MC, Cortes-Ortiz M et al (2014) Physiological role of amyloid Beta in neural cells: the cellular trophic activity, neurochemistry. In: Heinbockel T (ed). InTech. https://doi.org/10.5772/57398
Chasseigneaux S, Allinquant B (2012) Functions of Aβ, sAPPα and sAPPβ: similarities and differences. J Neurochem 120(Supl.1):99–108
Martínez-Morales PL, Revilla A, Ocaña I et al (2013) Progress in stem cell therapy for major human neurological disorders. Stem Cell Rev Rep 9(5):685–699
Lindvall O, Kokaia Z (2010) Stem cells in human neurodegenerative disorders—time for clinical translation? J Clin Invest 120(1):29–40
Martínez-Morales PL, Liste I (2012) Stem cells as in vitro model of Parkinson’s disease. Stem Cells Int 2012:980941. Epub 2012 Apr 30. PMID: 22619684
Villa A, Snyder EY, Vescovi A et al (2000) Establishment and properties of a growth factor-dependent, perpetual neural stem cell line from the human CNS. Exp Neurol 161(1):67–84
Villa A, Navarro-Galve B, Bueno C et al (2004) Long-term molecular and cellular stability of human neural stem cell lines. Exp Cell Res 294(2):559–570
Cummings BS, Schnellmann RG (2004) Measurement of cell death in mammalian cells. Curr Protoc Pharmacol. Chapter 12: Unit 12.8. doi: https://doi.org/10.1002/0471141755.ph1208s25
Porter AG, Jänicke RU (1999) Emerging roles of caspase-3 in apoptosis. Cell Death Differ 6(2):99–104
Bullwinkel J, Baron-Lühr B, Lüdemann A et al (2006) Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. J Cell Physiol 206(3):624–635
Lehner B, Sandner B, Marschallinger J et al (2011) The dark side of BrdU in neural stem cell biology: detrimental effects on cell cycle, differentiation and survival. Cell Tissue Res 345(3):313–328. https://doi.org/10.1007/s00441-011-1213-7
Acknowledgements
The authors wish to thank members of their laboratory for their research work and fruitful discussions. Research at the authors’ laboratory was funded by the MICINN-ISCIII (PI-10/00291 and MPY1412/09), MINECO (SAF2015-71140-R) and Comunidad de Madrid (NEUROSTEMCM consortium; S2010/BMD-2336).
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Bernabeu-Zornoza, A., Coronel, R., Lachgar, M., Palmer, C., Liste, I. (2018). Effects of Amyloid-β Peptide on the Biology of Human Neural Stem Cells. In: Sigurdsson, E., Calero, M., Gasset, M. (eds) Amyloid Proteins. Methods in Molecular Biology, vol 1779. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7816-8_23
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DOI: https://doi.org/10.1007/978-1-4939-7816-8_23
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