ATR-FTIR Analysis of Amyloid Proteins

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1777)

Abstract

Attenuated total reflection FTIR (ATR-FTIR) has been used for decades to study protein secondary structures. More recently, it reveals also to be an exquisite and sensitive tool to study and discriminate amyloid aggregates. Based on the analysis of specific spectral features of β-sheet structures, we present here a detailed protocol to differentiate oligomers vs. fibrils. This protocol, applicable to all amyloid proteins, demonstrates the power of this inexpensive, rapid, and low protein material-demanding method.

Key words

Amyloid Aggregates Oligomers Fibrils FTIR ATR Structure 

Abbreviations

ATR

Attenuated total reflection

FTIR

Fourier-transform infrared

FWHH

Full width at half height

HFIP

Hexafluoro-isopropanol

IRE

Internal reflection element

MCT

Mercury-Cadmium-Telluride

TFE

Trifluoroethanol

Notes

Acknowledgements

The authors would like to thank all the talented Ph.D. students and postdoctoral fellows who help to establish and improve this protocol. V.R. is Senior Research Associate for the National Fund for Scientific Research (F.R.S.-FNRS, Belgium). V.R. acknowledges funding from the F.R.S.-FNRS (PDR grant #70214.12) and SAO-FRA (grant S#14025).

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Science, Laboratory of Structure and Function of Biological Membranes, Center for Structural Biology and BioinformaticsUniversité Libre de BruxellesBrusselsBelgium

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