ATR-FTIR Analysis of Amyloid Proteins
Attenuated total reflection FTIR (ATR-FTIR) has been used for decades to study protein secondary structures. More recently, it reveals also to be an exquisite and sensitive tool to study and discriminate amyloid aggregates. Based on the analysis of specific spectral features of β-sheet structures, we present here a detailed protocol to differentiate oligomers vs. fibrils. This protocol, applicable to all amyloid proteins, demonstrates the power of this inexpensive, rapid, and low protein material-demanding method.
Key wordsAmyloid Aggregates Oligomers Fibrils FTIR ATR Structure
Attenuated total reflection
Full width at half height
Internal reflection element
The authors would like to thank all the talented Ph.D. students and postdoctoral fellows who help to establish and improve this protocol. V.R. is Senior Research Associate for the National Fund for Scientific Research (F.R.S.-FNRS, Belgium). V.R. acknowledges funding from the F.R.S.-FNRS (PDR grant #70214.12) and SAO-FRA (grant S#14025).
- 24.Harrick NJ (1967) Internal reflection spectroscopy. Wiley, New YorkGoogle Scholar