Programmable Fabrication of Multilayer Collagen Nanosheets of Defined Composition
Two-dimensional nanostructures offer significant promise as components for the construction of functional biomaterials. However, the controllable fabrication of these structures remains a challenge. Ideally, one desires to control the composition, structure, and surface functionality of the resultant materials with precision, in order to tailor properties for a particular application and minimize the unintended side effects. We recently reported the synthesis of triple-layer nanosheets from template-driven assembly of a negatively charged collagen-mimetic peptide CP − on a preassembled nanosheet of a positively charged collagen-mimetic peptide CP + . This process enabled the fabrication of nanosheets of defined composition, internal structure, and surface chemistry using a modified layer-by-layer approach. Herein, we describe the synthesis and purification procedures for these two 45-mer peptides, CP + and CP − , and guidelines for the directed assembly of triple-layer structures, along with routine methods of structural analysis.
Key wordsPeptides Self-assembly Collagen Nanosheets Triple helix
We acknowledge the National Science Foundation grant CHE-1012620 and CHE-1412580 for support.
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