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Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks

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Peptide Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1777))

Abstract

Recent advances in genetic engineering have provided a route to produce various types of recombinant spider silks. Different cloning strategies have been applied to achieve this goal (e.g., concatemerization, step-by-step ligation, recursive directional ligation). Here we describe recursive directional ligation as an approach that allows for facile modularity and control over the size of the genetic cassettes. This approach is based on sequential ligation of genetic cassettes (monomers) where the junctions between them are formed without interrupting key gene sequences with additional base pairs.

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Acknowledgments

We thank the NIH (P41 EB002520), the AFOSR, and the NSF for support of this work.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Tufts University, Medford, MA, USA

    Nina Dinjaski, Wenwen Huang & David L. Kaplan

Authors
  1. Nina Dinjaski
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  2. Wenwen Huang
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  3. David L. Kaplan
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Corresponding author

Correspondence to David L. Kaplan .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Rochester, Rochester, New York, USA

    Bradley L. Nilsson

  2. Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA

    Todd M. Doran

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Dinjaski, N., Huang, W., Kaplan, D.L. (2018). Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks. In: Nilsson, B., Doran, T. (eds) Peptide Self-Assembly. Methods in Molecular Biology, vol 1777. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7811-3_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7811-3_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7809-0

  • Online ISBN: 978-1-4939-7811-3

  • eBook Packages: Springer Protocols

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