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Virus-Derived Nanoparticles for Advanced Technologies pp 125–141Cite as

Production and Application of Insect Virus-Based VLPs

Part of the Methods in Molecular Biology book series (MIMB,volume 1776)

Abstract

Virus-like particles (VLPs) are self-assembling platforms composed of viral structural proteins. They are used for a variety of purposes, ranging from the study of virus assembly to vaccine development. VLPs can be produced in plants, bacteria, yeast, and insect and mammalian cells. The baculovirus expression system is one of the most commonly used systems for production of VLPs in eukaryotic cells. This chapter provides a brief overview of the main strategies used to generate recombinant baculoviruses and the applications of insect virus-derived VLPs in basic and applied research. It then describes detailed protocols for generation of recombinant baculoviruses, screening for their expression of VLPs in insect cells, and VLP purification.

Key words

  • Virus-like particle (VLP)
  • Baculovirus
  • Insect virus
  • Insect cells
  • Assembly
  • Vaccines

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Acknowledgment

This work was supported by NIH grant AI109081.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA

    Radhika Gopal & Anette Schneemann

Authors
  1. Radhika Gopal
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  2. Anette Schneemann
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Corresponding author

Correspondence to Anette Schneemann .

Editor information

Editors and Affiliations

  1. Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Dr. Christina Wege

  2. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    Prof. George P. Lomonossoff

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Gopal, R., Schneemann, A. (2018). Production and Application of Insect Virus-Based VLPs. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7808-3_8

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