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Virus-Derived Nanoparticles for Advanced Technologies pp 471–485Cite as

Presenting Peptides at the Surface of Potyviruses In Planta

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1776))

Abstract

Potyviruses are plant viruses with elongated, flexuous virions amenable to modifications in the only viral structural protein, the coat protein (CP). Out of the several theoretically possible modifications to the CP, the one most exploited for peptide presentation is the genetic fusion of the peptide-to-be-expressed, to the CP N-terminus. Successful high-level expression of the modified CP has been achieved this way. The purified recombinant viral particles incorporate most, if not all, the properties of the expressed peptides. For many purposes, the recombinant virus particles present in extracts of infected plants should be purified for further use. Procedures for carrying out the whole process, from cloning to purification are described in the chapter.

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Acknowledgments

The authors thank Ivonne González-Gamboa, Carmen Yuste-Calvo, Papaiah Sardaru, and Silvia López-González for their generous gift of graphic material. The technical support of Lucía Zurita is deeply acknowledged. Work about nanobiotechnological developments of TuMV has been funded over the years by several INIA grants.

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Authors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas (CBGP, UPM-INIA), Madrid, Spain

    Flora Sánchez & Fernando Ponz

Authors
  1. Flora Sánchez
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  2. Fernando Ponz
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Corresponding author

Correspondence to Fernando Ponz .

Editor information

Editors and Affiliations

  1. Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Christina Wege

  2. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    George P. Lomonossoff

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Sánchez, F., Ponz, F. (2018). Presenting Peptides at the Surface of Potyviruses In Planta. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_31

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  • DOI: https://doi.org/10.1007/978-1-4939-7808-3_31

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7806-9

  • Online ISBN: 978-1-4939-7808-3

  • eBook Packages: Springer Protocols

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